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  • Title: Unpredictability of triphenyltetrazolium chloride in staining irreversible ischaemia-reperfusion injury in the skeletal muscle of rats.
    Author: Cheng HT, Chang H, Lue HJ, Wen CY.
    Journal: Eur J Surg; 1996 May; 162(5):407-12. PubMed ID: 8781924.
    Abstract:
    OBJECTIVE: To find out whether pale staining with triphenyltetrazolium chloride (TTC) in skeletal muscle of rat hindlimbs which had been subjected to ischaemia-reperfusion definitely indicated irreversible tissue damage. DESIGN: Laboratory study. SETTING: University hospital, Taiwan. MATERIAL: 77 Female Wistar rats. INTERVENTIONS: Ischaemia of one hindlimb was caused by wrapping of a tourniquet above knee joint for 1.5 (n = 14). 2 (n = 15), 2.5 (n = 17), 3 (n = 17) or 4 (n = 14) hours. Each ischaemic group was divided into three subgroups to receive nil, 1 or 1.5 hours reperfusion, respectively. Gastrocnemius and soleus muscles were excised bilaterally. MAIN OUTCOME MEASURES: TTC reduction in the ischaemic limbs presented as a percentage of the opposite control limb measured by a spectrophotometric assay. Density of red formazan deposition in the ischaemic limbs assessed by microscopic examination of the TTC histochemical stain in the 3 hour ischaemic group. RESULTS: In the 2, 2.5, and 3 hour ischaemic groups the TTC reduction in the ischaemic limbs after 1.5 hours reperfusion increased significantly as compared with that with one hour reperfusion (p < 0.01 in each case). The density of red formazan deposition in the muscle after 3 hours ischaemia and 1.5 hours reperfusion was significantly higher than that of only 1 hour of reperfusion. CONCLUSION: Lack of TTC staining does not necessarily represent irreversible ischaemia-reperfusion injury of the skeletal muscle in rats.
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