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  • Title: Pyrimidine biosynthesis genes (pyrE and pyrF) of an extreme thermophile, Thermus thermophilus.
    Author: Yamagishi A, Tanimoto T, Suzuki T, Oshima T.
    Journal: Appl Environ Microbiol; 1996 Jun; 62(6):2191-4. PubMed ID: 8787418.
    Abstract:
    We have isolated uracil auxotrophic mutants of an extreme thermophile, Thermus thermophilus. A part of the pyrimidine biosynthetic operon including genes for orotate phosphoribosyltransferase (pyrE) and for orotidine-5'-monophosphate decarboxylase (pyrF) was cloned and sequenced. The pyrE gene can be a bidirectional marker for the gene manipulation system of the thermophile.
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