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  • Title: An improved method for the preparation of the DNA-dependent RNA polymerase B from calf thymus.
    Author: Grade K, Schönheit C, Lindigkeit R.
    Journal: Acta Biol Med Ger; 1977; 36(1):29-34. PubMed ID: 878742.
    Abstract:
    A simplified method is described for the large-scale preparation of a highly purified DNA-dependent RNA polymerase B from calf thymus. The method includes homogenization and lysis of the tissue, chromatography on DEAE-cellulose, phosphocellulose, hydroxylapatite-Sephadex G-10 and, once again, phosphocellulose. The procedure avoids the preparation of nuclei, the use of sonication, ammonium sulphate precipitations and dialysis steps and needs no ultracentrifugation.
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