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  • Title: A non-alpha-like DNA polymerase from the hyperthermophilic archaeon Pyrococcus furiosus.
    Author: Imamura M, Uemori T, Kato I, Ishino Y.
    Journal: Biol Pharm Bull; 1995 Dec; 18(12):1647-52. PubMed ID: 8787781.
    Abstract:
    We identified a DNA polymerase with properties that differed from those of alpha-like DNA polymerase (Pol I), in Pyrococcus furiosus, a hyperthermophilic archaeon. The novel DNA polymerase (Pol II) was partially purified and characterized. The DNA polymerizing activity of Pol II was relatively sensitive to dideoxythymidine-triphosphate (ddTTP) and it was inhibited by N-ethylmaleimide, but not by aphidicolin. Activity staining gel electrophoresis showed that the DNA polymerizing activity was derived from a polypeptide with a molecular weight of 130000 on sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis. The DNA elongation ability of Pol II using a natural DNA template was much lower than that of Pol I from this organism and DNA synthesis of Pol II seems to be non-processive.
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