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  • Title: Temporally resolved, independent stages of individual exocytotic secretion events.
    Author: Schroeder TJ, Borges R, Finnegan JM, Pihel K, Amatore C, Wightman RM.
    Journal: Biophys J; 1996 Feb; 70(2):1061-8. PubMed ID: 8789125.
    Abstract:
    The stages of the complex events involved in exocytotic secretion after vesicle-cell membrane fusion have been examined at the level of individual vesicles. Catecholamine flux from single bovine adrenal medullary cells was measured with carbon-fiber microelectrodes firmly touching the cell surface. The data reveal that secretion during exocytotic events has three distinct stages: a small increase in catecholamine flux, a rapid, but not instantaneous, rise to a maximum, followed by an exponential decrease in the flux. These stages are interpreted in the following ways. The initial stage corresponds to catecholamine secretion through a fusion pore. The rate of pore expansion appears to control the rise time of the flux to its maximum value. The final exponential stage is consistent with chemical dissociation of the intravesicular matrix or gel.
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