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  • Title: Polysaccharides from the seeds of Senna multijuga.
    Author: Rechia CG, Sierakowski MR, Ganter JL, Reicher F.
    Journal: Int J Biol Macromol; 1995 Dec; 17(6):409-12. PubMed ID: 8789348.
    Abstract:
    The seeds of Senna multijuga were extracted with water or 1% acetic acid and treated with ethanol, resulting in two insoluble fractions. After purification, the major one (FIA, 23%) was shown to be a galactomannan (Man:Gal 2.3:1; [alpha] = +54.6; [eta] = 1340 ml g-1). It consists of a main chain of (1-->4)-linked beta-D-mannopyranosyl residues substituted at O6 by single-unit alpha-D-galactopyranosyl side chains. The second fraction (FIB, 2.5%) was an O-acetyl-glucuronoarabinoxylan from the seed coats (O-acetyl 8.3 mol%; glucuronic acid 11.7%, Xyl:Ara ratio 20:1), which showed a predominance of 4-O-substituted Xylp units (84.4%), branched at O3 with non-reducing end units of Xylp, Araf and glucuronic acid. The O-acetyl positions in D-xylosyl units are at O2 (4.8%), O3 (4.4%) and O2,3 (0.9%). The ratio between O3 and O2 determined by 13C-nuclear magnetic resonance spectroscopy is 1.5:1.
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