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  • Title: Taurine- and glycine-conjugated bile acids in the preruminant goat: identification by high performance liquid chromatography.
    Author: Fernández JI, Mañas M, Rueda A, Martínez-Victoria E, Naranjo JA.
    Journal: Br Vet J; 1996 Jul; 152(4):433-9. PubMed ID: 8791851.
    Abstract:
    A reversed-phase high performance liquid chromatography technique has been developed for the identification of taurine- and glycine-conjugated bile acids in the bile of preruminant goats. The mobile phase consisted of two solvents: acetonitrile (A) and 5 mM potassium phosphate buffer (pH 4.5) (B). Samples (10 microliters) were eluted with a linear gradient in which acetonitrile was increased from 25 to 35%, and from 35 to 45%, at 10 min intervals. Flow rate was 1.0 ml min-1, and bile acids were detected at 200 nm. This sample high resolution technique was highly reproducible, involved a minimum of straightforward sample treatment, and required a short chromatographic development time. The technique will be of use in the systematic identifications of bile acids in preruminants.
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