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  • Title: A direct radioimmunoassay for estriol-16-glucuronide in urine for monitoring pregnancy and induction of ovulation.
    Author: Haning RV, Satin KP, Lynskey MT, Levin RM, Speroff L.
    Journal: Am J Obstet Gynecol; 1977 Aug 01; 128(7):793-802. PubMed ID: 879247.
    Abstract:
    Antibodies to estriol-16alpha-[beta-D-glucuronide] were raised in sheep with the use of keyhold limpet hemocyanin and bovine serum albumin conjugates of estriol-16alpha-[beta-D-glucuronide]. A simple, rapid method is presented for direct radioimmunoassay of estriol-16alpha-[beta-D-glucuronide] in urine with dextran-coated charcoal used for separation of free from bound and deionized water used for dilutions. The method is thrifty in its use of reagents. The assay has been evaluated in the pregnancy range, and the sensitivity has been extended into the range necessary for monitoring induction of ovulation with Pergonal. A direct radioimmunoassay technique for measuring estriol-16alpha-(beta-D-glucuronide) in pregnancy urine, and its application in the determination of estrogen production for monitoring the induction of ovulation with Pergonal, is reported. Antibodies to the estrogen were raised in sheep by the use of keyhole limpet hemocyanin and bovine serum albumin conjugates of estriol-16alpha-(beta-D-glucuronide). Dextron-coated charcoal was used to separate free conjugates from bound and deionized water used for dilutions. In monitoring the induction of ovulation by Pergonal, a significant (p less than .01) correlation was found between plasma estradiol and urinary estriol. The specificity of the method is evaluated.
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