These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: Fluorescence of spectrin-bound prodan.
    Author: Chakrabarti A.
    Journal: Biochem Biophys Res Commun; 1996 Sep 13; 226(2):495-7. PubMed ID: 8806662.
    Abstract:
    A large blue-shift (delta lambda max = 87 nm) in the fluorescence emission maximum and 2-fold enhancement in the emission intensity of PRODAN in the presence of 1.5 microM dimeric spectrin from the same aqueous buffer was observed. Binding of PRODAN to spectrin was estimated from the concentration-dependent change in the ratio of two fluorescence intensities, I520nm for the unbound and I430nm corresponding to the spectrin-bound PRODAN. PRODAN binds to spectrin with a higher affinity (Kapp = 2 X 10(6)M-1) than those reported for BSA and tubulin (Kapp = 1 X 10(5)M-1 for BSA and 5 X 10(4)M-1 for tubulin). This is the first report of PRODAN binding at such a low protein concentration.
    [Abstract] [Full Text] [Related] [New Search]