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  • Title: Role of mast cells, eosinophils and IL-5 in the development of airway hyperresponsiveness in sensitized mice.
    Author: Nagai H, Yamaguchi S, Maeda Y, Tanaka H.
    Journal: Clin Exp Allergy; 1996 Jun; 26(6):642-7. PubMed ID: 8809421.
    Abstract:
    BACKGROUND AND OBJECTIVE: In order to study the role of mast cells and IL-5 in allergen-induced airway hyperreactivity in mice, airway responsiveness in WBB6F1-W/Wv mice (mast cell deficient) and the effects of anti-IL-5 monoclonal antibody (NC-17) and three anti-allergic drugs (N-556, ketotifen and amlexanox) on airway hyperreactivity in Balb/c mice were studied. METHODS: Mice were immunized with an antigen (ovalbumin; OA) at intervals of 12 days. OA was inhaled 10 days after the secondary immunization. Twenty-four hours after the last inhalation, airway reactivity to acetylcholine was measured and bronchoalveolar lavage fluid (BALF) was obtained. RESULTS: Three inhalations of OA caused an increase in leucocytes (including eosinophils), accompanied by increases in IL-5 in BALF, and airway hyperreactivity to acetylcholine in Balb/c and WBB6F1- +/+ mice. In WBB6F1-W/Wv mice, antigen inhalation resulted in increases in leucocytes and IL-5 in BALF but did not result in airway hyperreactivity. NC-17 at doses between 10 and 20 micrograms (intratracheal injection) inhibited the antigen-induced eosinophilia but did not affect airway hyperreactivity in Balb/c mice. Three 'anti-allergic' drugs clearly inhibited antigen-induced increases in IL-5 levels and the number of eosinophils in BALF, but did not affect airway hyperreactivity in Balb/c mice. CONCLUSIONS: These data suggest that mast cells play an important role in the onset of airway hyperreactivity but do not play a role in the production of IL-5 and eosinophilia. Furthermore, indicate that the inhibition of IL-5 is not always associated with a reduction in antigen-induced airway hyperreactivity in mice.
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