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  • Title: One-day detection of PCR amplified Chlamydia trachomatis DNA in clinical samples: ELISA versus Southern blot hybridisation.
    Author: Roymans RT, Onland G, Postma BH.
    Journal: J Clin Pathol; 1996 Jul; 49(7):581-3. PubMed ID: 8813959.
    Abstract:
    AIMS: To compare ELISA and Southern blot hybridisation for the detection of PCR amplified Chlamydia trachomatis DNA extracted from clinical samples; to assess the value of the ELISA method in a clinical setting. METHODS: DNA was extracted from urogenital samples of 508 patients, purified and amplified using C trachomatis specific primers, one of which was endlabelled with biotin. Amplification products were detected by Streptavidin biotin based ELISA and non-radioactive Southern blotting. RESULTS: Of the 508 samples, 29 were positive and 479 negative by both methods. No discrepant results were observed. CONCLUSION: Streptavidin biotin based ELISA and Southern blotting were equally sensitive for detecting PCR amplified C trachomatis DNA. Using ELISA, test results could be generated within a single day.
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