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  • Title: Development and validation of a high-performance liquid chromatography method for the determination of cocaine, its metabolites and lidocaine.
    Author: Barat SA, Kardos SA, Abdel-Rahman MS.
    Journal: J Appl Toxicol; 1996; 16(3):215-9. PubMed ID: 8818861.
    Abstract:
    Street cocaine varies in purity and is often adulterated with various compounds. Some of these additives, such as lidocaine, may increase the toxicity of cocaine. A new precise, accurate and sensitive reversed-phase high-performance liquid chromatography method for the determination of cocaine, its metabolites and lidocaine in plasma samples has been developed and validated. This assay employed a phosphate-buffered aqueous mobile phase (pH 6.0) with an organic component consisting of acetonitrile and methanol and a C-18 column as the stationary phase. Minimum detection limits were 1 ng ml-1 for cocaine, 2.5 ng ml-1 for ethylcocaine and 5 ng ml-1 for benzoylecgonine, norcocaine, norethylcocaine and lidocaine. Linearity was demonstrated over a broad range of concentrations in plasma, with good sensitivity for cocaine and cocaine derivatives.
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