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  • Title: Nucleotide sequence determination and genetic analysis of the Bacteroides plasmid, pBI143.
    Author: Smith CJ, Rollins LA, Parker AC.
    Journal: Plasmid; 1995 Nov; 34(3):211-22. PubMed ID: 8825374.
    Abstract:
    The nucleotide sequence and genetic organization of the Bacteroides plasmid pBI143 were determined. The plasmid was 2747 base pairs (bp) and had a G+C content of 41% (GenBank Accession No. U30316). There were two open reading frames greater than 50 codons and these were designated mobA and repA. A 56-bp inverted repeat divided pBI143 into modules with repA and mobA in separate regions. There was a marked difference in the G+C content and codon usage for the two regions; repA had 33% G+C and mobA was 44% G+C. MobA had homology to other Bacteroides mobilization proteins and RepA shared homology to a replication protein from Zymomonas mobilis plasmid pZM2. These two putative replication proteins formed a subgroup of the rolling-circle replication.proteins belonging to the pSN2 family of gram-positive plasmids. Consistent with this finding, single-stranded pBI143 DNA was detected in plasmid containing Bacteroides fragilis cultures. Availability of the pBI143 sequence allowed the elucidation of the complete nucleotide sequence for pFD288 an 8.9-kb Bacteroides shuttle vector (GenBank Accession No. U30830).
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