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  • Title: Effect of 5-hydroxytryptamine on bioelectric properties of airway epithelial cells in culture.
    Author: Tamaoki J, Chiyotani A, Takemura H, Konno K.
    Journal: Res Commun Mol Pathol Pharmacol; 1996 Jun; 92(3):285-98. PubMed ID: 8827827.
    Abstract:
    To elucidate whether 5-hydroxytryptamine (5-HT) affects airway epithelial electrolyte transport and, if so, what the mechanism of action is, we studied the bioelectric properties of canine cultured tracheal epithelium under short-circuit conditions in vitro. Mucosal addition of 5-HT dose-dependently increased short-circuit current (Isc), which was accompanied by increases in transepithelial potential difference and cell conductance. In contrast, 5-HT had no effect on bioelectric properties when it was added to the submucosal side. Pretreatment of cells with amiloride potentiated the increase in Isc produced by 5-HT. In the presence of diphenylamine-2-carboxylate or Cl-free medium, 5-HT decreased Isc from the baseline level. Incubation with BAPTA-AM but not dibutyryl cAMP greatly attenuated the 5-HT-induced increase in amiloride-insensitive portion of Isc. These results suggest that 5-HT inhibits Na absorption and stimulates Cl secretion across canine tracheal epithelium and that the Cl secretion may be associated with elevation of intracellular Ca2+ contents.
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