These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: Assessment of variability in some lysosomal and soluble enzyme activities in corneal epithelium from slaughterhouse bovine eyes.
    Author: Doughty MJ.
    Journal: Ophthalmic Res; 1996; 28(3):141-6. PubMed ID: 8829169.
    Abstract:
    The corneal epithelium was scraped from bovine eyes (Hereford or Holstein) within 3 h post mortem. The mitochondriallysosomal fraction of the cells was assayed for hexosaminidase (HEX) and acid phosphatase (AP), and the membrane-soluble fraction for lactate dehydrogenase (LDH) and leucine arylamidase (LAA) activity. Pairs of eyes with clear corneas showed variances (+/-1 SD, n = 10 pairs) of +/-62, +/-57, +/-72 and +/-31% in the four enzyme activities (HEX, AP, LDH, LAA). Selection based on age (corneal diameter) reduced the variance to +/-41, +/-37, +/-39 and +/-30%. Additional selection for corneal surface wettability reduced variances to +/-26, +/-21, +/-22 and +/-26% in the activities. The latency of the lysosomal enzymes (HEX, AP) increased substantially with eye selection.
    [Abstract] [Full Text] [Related] [New Search]