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  • Title: Direct application of Etest to gram-positive cocci from blood cultures: quick and reliable minimum inhibitory concentration data.
    Author: Hong T, Ndamukong J, Millett W, Kish A, Win KK, Choi YJ.
    Journal: Diagn Microbiol Infect Dis; 1996 May; 25(1):21-5. PubMed ID: 8831041.
    Abstract:
    The Etest was applied directly to 100 Gram-positive bacterial strains from blood cultures to measure their minimum inhibitory concentration (MIC). Results showed 100% concordance of MIC data between a direct Etest method and the standard Etest method for Streptococcus pneumoniae, beta-hemolytic streptococci, and viridans group streptococci. In addition, direct Etest for Staphylococcus aureus, coagulase negative staphylococci, Enterococcus faecium, and Enterococcus faecalis showed 83 to 100% correlation with standard Etests. These data indicate that the Etest is useful to obtain MIC data on Gram-positive cocci (especially streptococci) directly from positive blood cultures. The advantages of a direct Etest are two fold: MIC results can be obtained 24 hours earlier than standard methods and a more representative population of the bacterial isolate is tested.
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