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  • Title: Theory of gel electrophoresis of DNA: orientational effects.
    Author: Subbotin AV, Semenov AN.
    Journal: Electrophoresis; 1996 Jun; 17(6):1018-26. PubMed ID: 8832167.
    Abstract:
    Some aspects of the reptation model of gel electrophoresis of DNA, with emphasis on the orientational effects, are considered. A master equation for the reptational dynamics with the end boundary conditions is proposed. Based on this equation, the orientation and dynamics of the persistent-chain are investigated. In particular, the role of trap conformations for this chain, which essentially reduce the chain mobility, was analyzed. A transient orientation of the chain during pulsed field gel electrophoresis is also discussed.
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