These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: Demonstration of an upper limit to the range of association rate constants amenable to study by biosensor technology based on surface plasmon resonance.
    Author: Hall DR, Cann JR, Winzor DJ.
    Journal: Anal Biochem; 1996 Mar 15; 235(2):175-84. PubMed ID: 8833325.
    Abstract:
    Numerical simulation of BIAcore sensorgrams has highlighted the need for concern about an assumption, inherent in current determinations of rate constants for macromolecular interactions, that the concentration of solute in the flowing phase remains constant at its injected value. This assumption is shown to be valid for systems with effective association rate constants equal to or less than 10 M(-1) values characteristic of antibody interactions with protein antigens. However, the assumption loses validity when the effective association rate constant is raised to 10 M' . The basic correctness of the latter prediction is verified by an experimental study of the interaction between soybean trypsin inhibitor and immobilized -trypsin, a system with comparable reaction kinetics.
    [Abstract] [Full Text] [Related] [New Search]