These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: Anion-selectivity of the swelling-activated osmolyte channel in eel erythrocytes.
    Author: Lewis RA, Bursell JD, Kirk K.
    Journal: J Membr Biol; 1996 Jan; 149(2):103-11. PubMed ID: 8834117.
    Abstract:
    Osmotic swelling of fish erythrocytes activates a broad-specificity permeation pathway that mediates the volume-regulatory efflux of taurine and other intracellular osmolytes. This pathway is blocked by inhibitors of the erythrocyte band 3 anion exchanger, raising the possibility that band 3 is involved in the volume-regulatory response. In this study of eel erythrocytes, a quantitative comparison of the pharmacology of swelling-activated taurine transport with that of band 3-mediated SO4(2-) transport showed there to be significant differences between them. N-ethylmaleimide and quinine were effective inhibitors of swelling-activated taurine transport but caused little, if any, inhibition of band 3. Conversely, DIDS was a more potent inhibitor of band 3-mediated SO4(2-) flux than of swelling-activated taurine transport. In cells in isotonic medium, pretreated then co-incubated with 0.1 mM DIDS, the band 3-mediated transport of SO4(2-) and Cl- was reduced to a low level. Exposure of these cells to a hypotonic medium containing 0.1 mM DIDS was followed by the activation of a Cl- permeation pathway showing the same inhibitor sensitivity as swelling-activated taurine transport. The data are consistent with swelling-activated transport of taurine and Cl- being via a common pathway. A comparison of the swelling-activated transport rates for taurine and Cl- with those for several other solutes was consistent with the hypothesis that this pathway is an anion-selective channel, similar to those that mediate the volume-regulatory efflux of Cl- and organic osmolytes from mammalian cells.
    [Abstract] [Full Text] [Related] [New Search]