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  • Title: Effects of prenatal morphine and adult estrogen administration on mu-opioid inhibition of norepinephrine release from hypothalamic slices.
    Author: Vathy I, Etgen AM.
    Journal: Neuroendocrinology; 1996 Jan; 63(1):61-8. PubMed ID: 8839356.
    Abstract:
    The present study tested the hypotheses that exposure to morphine in utero (10 mg/kg twice a day on gestation days 11-18) and acute estrogen treatment in adulthood alter mu-opioid regulation of hypothalamic norepinephrine (NE) release in sexually mature rats. Both basal and KCl-stimulated NE releases were measured in superfused hypothalamic and preoptic area (POA) slices preloaded with 3H-NE. The mu-opioid receptor agonist D-Ala2, MePhe4-Gly-ol5-enkephalin (DAMGO; 10 or 100 nM) or the opioid antagonist naloxone (1 microM) was applied to some slices 15 min prior to KCl stimulation. Prenatal morphine had no effect on basal or KCl-stimulated release of preloaded 3H-NE from hypothalamic and POA slices from adult male progeny. Neither the mu-opioid agonist DAMGO nor the nonspecific antagonist naloxone significantly affected KCl-evoked overflow of 3H-NE in slices from either brain region of male rats. Adult female offspring were ovariectomized (OVX), and some were injected with a replacement dose of estrogen 48 h prior to sacrifice. Prenatal morphine had no effect on basal or KCl-stimulated release of 3H-NE from hypothalamic or POA slices or on the response of slices to opioid drugs. Estrogen treatment modestly increased KCl-evoked release of 3H-NE from POA slices from females. Moreover, there was a significant interaction between opioid drugs and estrogen treatment on KCl-evoked overflow of 3H-NE. In hypothalamic and POA slices from OVX females, DAMGO reduced KCl-evoked efflux of 3H-NE. This inhibitory effect of DAMGO was not apparent in slices from estrogen-treated females. In addition, naloxone increased KCl-stimulated NE release in slices from both hypothalamus and POA of estrogen-treated female rats but not control OVX females. Thus, prenatal exposure to morphine does not alter basal, KCl-evoked or mu-opioid modulation of NE efflux from hypothalamic or POA slices in adult progeny of either sex. However, treatment of adult, OVX females with estrogen attenuates mu-opioid inhibition and promotes the appearance of naloxone facilitation of KCl-evoked 3H-NE release from hypothalamic and POA slices.
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