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  • Title: Chimerism analysis after allogeneic bone marrow transplantation with nonradioactive RFLP and PCR-AFLP using the same DNA.
    Author: Schreiner T, Prochnow-Calzia H, Maccari B, Erne E, Kinzler I, Wölpl A, Wiesneth M.
    Journal: J Immunol Methods; 1996 Sep 13; 196(1):93-6. PubMed ID: 8841447.
    Abstract:
    We describe a method combining RFLP and PCR-AFLP analyses for studying chimerism after allogeneic bone marrow transplantation. Using RFLP analysis alone with DNA probe hMFl 87% of 244 donor/recipient pairs revealed different specific bands. By examination of the identical DNA probes using PCR-AFLP, an additional 52% of the residual donor/recipient pairs could be identified. Thus, 94% of allogeneic transplantations can be monitored using a combination of RFLP and PCR-AFLP analyses at one locus.
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