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  • Title: Irreversible inactivation of Caldariomyces fumago chloroperoxidase by hydrogen peroxide. A kinetic study in chloride and bromide system.
    Author: Shevelkova AN, Ryabov AD.
    Journal: Biochem Mol Biol Int; 1996 Jul; 39(4):665-70. PubMed ID: 8843333.
    Abstract:
    The rate constants for the H2O2-induced irreversible inactivation (kinact) of chloroperoxidase from Caldariomyces fumago evaluated from the analysis of complete kinetic curves of chlorination or bromination of monochlorodimedon were found to follow the rate law kinact = k[H2O2]/(K + [H2O2]) with k = 0.009 > or = 0.002 and 0.0095 > or = 0.010 s-1 and K = (13 > or = 4) x 10(-3) and (9 > or = 2) x 10(-3) M in the presence of 0.01 M chloride and bromide, respectively, at pH 2.75 and 25 degrees C. The data show that chloroperoxidase investigated is more than by a factor of 10 less resistant toward hydrogen peroxide compared to horseradish peroxidase. The possible reason for it and the biotechnological implications are briefly discussed.
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