These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: Super-sensitive enzyme immunoassay for thyroid stimulating hormone using a new synergistic enhanced chemiluminescent endpoint.
    Author: Kricka LJ, Ji X.
    Journal: J Biolumin Chemilumin; 1996; 11(3):137-47. PubMed ID: 8844344.
    Abstract:
    The enhancers 1,1'-biphenyl-4-yl boronic acid and 4-iodophenol act synergistically in the horseradish peroxidase-catalysed oxidation of luminol. This concentration-dependent effect reduces background, increases signal and hence improves signal/background for detection of peroxidase. The same type of synergistic effect was found when 1,1'-biphenyl-4-yl boronic acid was added to a commercial enhanced chemiluminescence signal reagent (Amerlite Signal Reagent). This synergistic enhanced chemiluminescent endpoint (Amerlite Signal Reagent containing 1,1'-biphenyl-4-yl boronic acid) for a horseradish peroxidase label has been tested in the Amerlite TSH and the Amerlite TSH-30 Ultrasensitive assays. The detection limit (mean of 20 replicates of the zero standard+2SD) in the Amerlite TSH assay was 0.0029 mIU/L, and in the Amerlite TSH-30 Ultrasensitive assay the detection limit was 0.0005 mIU/L using the synergistic enhanced endpoint. Reassessment of the detection limit using a 1:40 dilution of the first standard (0.119 mIU/L) as the lowest assay standard gave a value of 0.0015 mIU/L for the Amerlite TSH-30 Ultrasensitive assay with the synergistic endpoint. A limited (n = 29) method comparison using samples from euthyroid, hyperthyroid and hypothyroid patients revealed excellent correlation between the conventional and synergistic TSH immunoassays.
    [Abstract] [Full Text] [Related] [New Search]