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  • Title: Determination of glibenclamide in human plasma by solid-phase extraction and high-performance liquid chromatography.
    Author: Valdes Santurio JR, González Porto E.
    Journal: J Chromatogr B Biomed Appl; 1996 Jul 12; 682(2):364-70. PubMed ID: 8844432.
    Abstract:
    A sensitive high-performance liquid chromatographic method for determination of intact glibenclamide in human plasma has been developed. Sample clean-up prior to chromatographic analysis was accomplished by extraction of the drug using a solid-phase RP-8 or RP-18 cartridge instead of the conventional liquid-liquid extraction methods described. For the separation of the drug from the endogenous components a reversed-phase column (LiChrosorb RP-8) of 5 microns particle size and 250 x 4 mm I.D., together with a mobile phase consisting of aceronitrile-12 mM perchloric acid (47:53) was selected. The method employs progesterone as an internal standard, and a reversed-phase column combined with UV detection of the drug at 230 nm. The detector response was linear up to the concentration of 400 ng/ml and the average recovery was 100.36%. The sensitivity of the method was 5 ng/ml.
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