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  • Title: Enzymic preparation of adenosine 5'-[beta-2P]triphosphate.
    Author: Leung KL, Yamazaki H.
    Journal: Can J Biochem; 1977 Mar; 55(3):23-6. PubMed ID: 884581.
    Abstract:
    A simple method for the preparation of adenosine 5'-[beta-32 P]triphosphate is described. When [32P]orthophosphate was incubated with polyadenylate and phosphoenolpyruvate in the presence of polynucletide phosphorylase (EC 2.7.7.8) and pyruvate kinase (EC 2.7.1.40), up to 75% of 32P radioactivity was recovered in ATP. [32P]ATP was purified to 99.5% radiochemical purity by chromatography on polyethyleneimine-cellulose thin-layer plates. Analysis of hydrolysis products of [32P]ATP with apyrase (EC 3.6.1.5) indicates that 32P in the beta-phosphate position accounts for all 32P label in ATP.
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