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  • Title: Analytical determination of fumonisins and other metabolites produced by Fusarium moniliforme and related species on corn.
    Author: Plattner RD, Weisleder D, Poling SM.
    Journal: Adv Exp Med Biol; 1996; 392():57-64. PubMed ID: 8850605.
    Abstract:
    Fumonisins, secondary metabolites of the fungus Fusarium moniliforme are potent toxins that can be found in fungal contaminated corn. The detection and measurement of these toxins by HPLC with detection by an evaporative light scattering detector and by electrospray MS is reported. The light scattering detector had enough sensitivity to analyze culture materials, however, clean-up was necessary to detect fumonisins at sub-ppm levels in naturally contaminated corn extracts. The detection limit for FB1 with the light scattering detector was in the low ng range (10-50) while the detection limit of less than 1 ng injected was observed for the electrospray detector. Several previously unreported fumonisin isomers were observed in electrospray chromatograms of culture extracts. Two of these compounds, FA3 and FA4 were isolated and their proposed structure confirmed by NMR experiments.
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