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  • Title: Production and purification of Japanese quail ovalbumin as fusion protein with glutathione S-transferase in Escherichia coli.
    Author: Visvaderová J, Albert S, Kosová A, Klaudiny J, Simúth J.
    Journal: Folia Microbiol (Praha); 1995; 40(2):169-75. PubMed ID: 8851561.
    Abstract:
    A plasmid encoding a fusion protein interlinked by thrombin recognition sequence between glutathione S-transferase and Japanese quail ovalbumin (without 40 amino acid residues from the 5'-end of the ORF) has been constructed, employing the expression system pGEX-2T. The deglycosylated fusion protein (64 kDa) was purified by affinity chromatography on glutathione agarose beads, analyzed by SDS-polyacrylamide gel electrophoresis, immunochemically detected with antiserum raised against Japanese quail ovalbumin and tested for its stability.
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