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  • Title: The expression of estrogen receptor and estrogen effect in MBA-15 marrow stromal osteoblasts.
    Author: Shamay A, Knopov V, Benayahu D.
    Journal: Cell Biol Int; 1996 Jun; 20(6):401-5. PubMed ID: 8858824.
    Abstract:
    MBA-15, a marrow stromal-derived cell line, was shown to express an estrogen receptor. This finding was confirmed by in situ hybridization and receptor binding assay. An exposure to estrogen (10(-12)-10(-6) M) in a dose response manner resulted in a decrease of cell proliferation as measured by MTT assay. Cell function was measured by enzymatic activities of two osteoblastic markers, CD10/NEP and alkaline phosphatase. These enzymatic activities were elevated following the estrogen treatment. This model enabled direct evaluation of the estrogen effect on stromal osteoblast cells.
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