These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: Purification of beta-N-acetyl-D-glucosaminidase of the horseshoe crab by affinity chromatography.
    Author: Jain RS, Binder RL, Walz C, Buck CA, Warren L.
    Journal: J Chromatogr; 1977 Jun 01; 136(1):141-6. PubMed ID: 885954.
    Abstract:
    An affinity column has been synthesized consisting of p-aminophenyl 1-thio-beta-L-fucopyranoside residues attached to Sepharose 4B through succinylated diaminodipropylamine bridges. Surprisingly, it has been found to bind beta-N-acetylglucosaminidase in the serum of Limulus polyphemus (horseshoe crab). The enzyme is eluted with N-acetyl-D-glucosamine at a concentration of 2 mg/ml and with other sugars at higher concentrations. A highly purified enzyme free from other glycosidases is obtained. The enzyme is not eluted by solutions of salt.
    [Abstract] [Full Text] [Related] [New Search]