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  • Title: Preparative countercurrent chromatography with a slowly rotating helical tube.
    Author: Ito Y, Bowman RL.
    Journal: J Chromatogr; 1977 Jun 11; 136(2):189-98. PubMed ID: 885964.
    Abstract:
    The capability of a simple countercurrent chromatographic scheme for obtaining high-resolution preparative-scale separations was demonstrated with the separations of a series of dinitrophenyl (DNP) amino acids and peptides. Basic studies on stationary phase retention and partition efficiency with a low-viscosity chloroform-acetic acid-0.1 N hydrochloric acid (2:2:1) phase system together with the results previously obtained with a vascous n-butanol system suggest a general applicability of low-interfacial tension phase systems by the present method.
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