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  • Title: Inhibition of in vitro limb cartilage differentiation by syndecan-3 antibodies.
    Author: Seghatoleslami MR, Kosher RA.
    Journal: Dev Dyn; 1996 Sep; 207(1):114-9. PubMed ID: 8875081.
    Abstract:
    The transmembrane heparan sulfate proteoglycan syndecan-3 is transiently expressed in high amounts during the cellular condensation process that characterizes the onset of limb cartilage differentiation. During condensation, limb mesenchymal cells become closely juxtaposed and undergo cell-cell and cell-matrix interactions that are necessary to trigger cartilage differentiation and cartilage-specific gene expression. To test directly the possible involvement of syndecan-3 in regulating the onset of limb chondrogenesis, we examined the effect of polyclonal antibodies against a syndecan-3 fusion protein on the chondrogenic differentiation of chick limb mesenchymal cells in micromass culture. Syndecan-3 antiserum elicits a dose-dependent inhibition of the accumulation of Alcian blue-stainable cartilage matrix by high density limb mesenchymal cell micromass cultures (2 x 10(5) cells/10 microliters) and a corresponding reduction in steady-state levels of mRNAs for cartilage-characteristic type II collagen and the core protein of the cartilage proteoglycan aggrecan. In preimmune serum-treated control cultures proliferating cells are limited to the periphery of areas of cartilage matrix deposition, whereas large numbers of proliferating cells are uniformly distributed throughout the undifferentiated cultures supplemented with syndecan-3 antiserum. Limb mesenchymal cells cultured at lower densities (1 x 10(5) cells/10 microliters) in the presence of preimmune serum form extensive precartilage condensations characterized by the close juxtaposition of rounded cells by day 2 of culture. In contrast, in the presence of syndecan-3 antiserum, the cells fail to aggregate but rather remain flattened and spatially separated from one another, suggeting that syndecan-3 antibodies impair the formation of precartilage condensations. These results indicate that syndecan-3 plays an important role in regulating the onset of limb chondrogenesis, perhaps by mediating the cell-cell and cell-matrix interactions required for condensation and subsequent cartilage differentiation.
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