These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: Modulation of ANF-R2/ANP-C receptors by angiotensin II in vascular smooth muscle cells.
    Author: Palaparti A, Anand-Srivastava MB.
    Journal: Am J Hypertens; 1996 Sep; 9(9):930-4. PubMed ID: 8879351.
    Abstract:
    In the present studies, we have investigated the modulation of atrial natriuretic factor (ANF) receptor of R2 subtype (ANF-R2) coupled to adenylyl cyclase/cAMP signal transduction system by angiotensin II (AII). C-ANF4-23 [C-ANF4-23, [des(Gln18, Ser19, Gln20, Leu21, Gly22)ANF4-23-NH2] and AII inhibited adenylyl cyclase activity in control vascular smooth muscle cells (VSMC A-10) by about 40% and 30% respectively. Pretreatment of the cells with AII resulted in the attenuation of both C-ANF4-23- and AII-mediated inhibition of adenylyl cyclase. Losartan treatment of the cells was able to partially block (approximately 50%) the AII- as well as C-ANF4-23-mediated inhibitions of adenylyl cyclase that are completely lost by AII pretreatment. The pretreatment of the cells with AII alone or with losartan did not affect the [125I]-ANF binding to ANF receptors. However, AII treatment resulted in the augmentation of the levels of Gi alpha 2 and Gi alpha 3. On the other hand, staurosporine (a protein kinase C [PKC] inhibitor) treatment of cells before AII treatment was able to prevent the attenuation of both C-ANF4-23 as well as AII-mediated inhibition of adenylyl cyclase elicited by AII. These results indicate that the AII modulation of ANF-R2 receptor-mediated inhibition of adenylyl cyclase is independent of ANF-R2 receptor density or the levels of Gi regulatory protein and may be due to the uncoupling of the ANF-R2 receptor from the Gi protein. This uncoupling may be associated with the phosphorylation of the Gi protein by PKC activated by AII.
    [Abstract] [Full Text] [Related] [New Search]