These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: Fertility of ram spermatozoa pellet-frozen in zwitterion-buffered diluents.
    Author: Molinia FC, Evans G, Maxwell WM.
    Journal: Reprod Nutr Dev; 1996; 36(1):21-9. PubMed ID: 8881589.
    Abstract:
    The fertility (positive ultrasound scanning 60-75 days after insemination) of ram spermatozoa frozen in Tris- or zwitterion/glucose/egg yolk/glycerol-based diluents was examined during the natural breeding season (autumn). In Experiment 1 Tris-citrate, high glucose and Hepes-NaOH diluents were used and ewes (n = 266) were inseminated (induced oestrus) with 20 (uterus) or 180 (cervix) million motile spermatozoa. There was no difference in fertility (range: 10.9-71.1%) between diluents, despite the superior post-thawing motility and progressive velocity of spermatozoa in Hepes-NaOH compared with the other diluents (P < 0.001). In Experiment 2, there was no difference in fertility (range: 4.1-50.0%) between Tris-citrate and Hepes-NaOH diluents after insemination (induced oestrus) of ewes (n = 290) with 10 (uterus) or 100 (cervix) million motile spermatozoa, respectively. In Experiment 3 Tris-citrate, Hepes-NaOH and Pipes-NaOH diluents were used and ewes (n = 492) were inseminated (natural oestrus) with 8 or 24 (uterus), or 80 or 240 (cervix) million motile spermatozoa. There were no differences in fertility (range: 35.4-52.9%) between insemination doses or diluents despite the superior motility and acrosome integrity in the zwitterion compared with the Tris-citrate diluents (P < 0.001). In all experiments, the fertility of frozen spermatozoa was higher for intrauterine than cervical insemination (51.9 vs 22.1%, n = 1 048; P < 0.01). It was concluded that post-thawing in vitro quality was superior and the fertility comparable after freeze-thawing spermatozoa in zwitterion compared with Tris-citrate diluents.
    [Abstract] [Full Text] [Related] [New Search]