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  • Title: In vivo monitoring of .OH generation on jejunal ischemic injury by dialysis technique.
    Author: Hirata T, Obata T, Yamanaka Y, Uchida Y.
    Journal: Res Commun Mol Pathol Pharmacol; 1996 Aug; 93(2):187-97. PubMed ID: 8884990.
    Abstract:
    We applied an in vivo microdialysis technique to examine free radical generation following ischemic injury in the rat jejunum. For this purpose, we used a microdialysis probe holding system. The salicylate reacts with hydroxyl free radical (.OH) and generates 2,3-dihydroxybenzoic acid (DHBA), which can be measured electrochemically in picomole amounts by high-performance liquid chromatography (HPLC). Therefore, the former was used for trapping the .OH formation. Reperfusion of the jejunum produced progressive elevations in the levels of 2,3-DHBA achieving the maximums at 20 min, after which they decreased progressively to the baseline. These elevations were significant only after 10 min- and 20 min-ischemia. However, this change was not significant in the case of 30 min-ischemia. Ischemia-reperfusion of rat intestine caused morphological changes. These results suggest that .OH production is significantly reduced after 30 min-ischemia providing thus the possibility for more severe intestinal damage. In conclusion our dialysis probe can be useful for the measurement of .OH generation following ischemia-reperfusion injury.
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