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  • Title: Interactions of cytosine derivatives with T.A interruptions in pyrimidine.purine.pyrimidine DNA triplexes.
    Author: Verma S, Miller PS.
    Journal: Bioconjug Chem; 1996; 7(5):600-5. PubMed ID: 8889023.
    Abstract:
    The ability of triplex-forming oligopyrimidines to interact with duplex targets which contain a single pyr.pur interruption in their homopurine triplex binding site was studied. These oligonucleotides contain either N4-(3-carboxypropyl)deoxycytidine (3) or N4-(5-carboxytriazolyl)deoxycytidine (4) to target the pyr.pur interruption. The 3-carboxypropyl and the 5-carboxytriazolyl groups of these cytosine derivatives are designed to span the major groove of the duplex target. Molecular models suggest that the carboxyl group of 3 or 4 can serve as a hydrogen bond acceptor for the N6-amino hydrogen of A in a T.A base pair. Additional contacts are possible between the N4-amino hydrogen of 3 or 4 and the O4-carbonyl oxygen of T. UV melting experiments showed that a 15-mer, I(3), containing nucleoside 3 formed a stable triplex with duplex targets containing a single T.A interruption. The melting temperature of this triplex was 7 degrees C higher than that of a similar triplex containing a single G.T.A triad when a Tris buffer was employed. Oligomer I(3) also formed a triplex of lower stability with a target containing a G.C base pair but not with targets containing C.G, U.A, or A.T base pairs. A similar 15-mer, II, containing nucleoside 4 was found to be less selective than I(3) in its interaction with duplex targets. Thus, gel mobility shift experiments showed that in addition to interacting with T.A, oligomer II also formed triplexes with duplex targets containing U.A and C.G interruptions. These studies suggest that nucleoside derivatives which can potentially contact both bases of a pyr.pur interruption might provide a means to extend the range of sequences which can be recognized by triplex-forming oligonucleotides.
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