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  • Title: Sequence analysis of the 2,4-dichlorophenol hydroxylase gene tfdB and 3,5-dichlorocatechol 1,2-dioxygenase gene tfdC of 2,4-dichlorophenoxyacetic acid degrading plasmid pEST4011.
    Author: Kõiv V, Marits R, Heinaru A.
    Journal: Gene; 1996 Oct 03; 174(2):293-7. PubMed ID: 8890750.
    Abstract:
    Chlorocatechol 1,2-dioxygenase (CC12O) and 1,2-dichlorophenol hydroxylase (DCPH) encoding genes tfdC and tfdB are located on a 4.2-kb DNA fragment cloned from the 2,4-dichlorophenoxyacetic acid (2,4D) degrading plasmid pEST4011. The nucleotide sequences of tfdC and tfdB were determined. The DCPH is coded by a 1758-bp gene and CC12O is coded by a 762-bp gene. The deduced M(r) of these proteins are 64.09 kDa and 28.2 kDa, respectively. Expression analysis of tfdB and tfdC in Escherichia coli suggested that these genes form one operon, tfdCB.
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