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  • Title: Dynamic adhesion of erythrocytes to glass. Comparative study of the influence of serum in low concentrations.
    Author: Kowalczyńska HM.
    Journal: Arch Immunol Ther Exp (Warsz); 1977; 25(3):397-408. PubMed ID: 889429.
    Abstract:
    Adhesion of 51Cr-labeled rat erythrocytes was examined in a layer of glass beads in a dynamic system. The kinetics of erythrocyte retention in the bead layer was found to change with time. Adhesion decreased with time at all the studied rates of suspension flow through the layer (within the range 1-5--0-4 cm/min), both in presence and absence of serum in the medium. Serum in low concentrations of 20--200 microgram of protein per ml considerably inhibited dynamic adhesion of erythrocytes to glass. The higher the serum concentration in a medium (at a definite flow velocity), the quicker the decrease of adhesion with time, and the stronger the dependence of the kinetics of erythrocyte retention on the flow velocity of the cells. Mean adhesion of erythrocytes after 17-min perfusion of the layer (at 200 microgram of serum protein per ml of medium) seems to be independent of the concentration of the cells in the suspension flowing into the layer within the range of studied concentrations, 2 x 10(6)--20 x 10(6) cells/ml. However, mean adhesion of erythrocytes deprived of serum seems to be dependent on the cell concentration.
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