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  • Title: Cold preservation-induced changes in oxygen radical generation between parenchymal and nonparenchymal cells in rat liver.
    Author: Sato B, Tanaka A, Mori S, Yanabu N, Kitai T, Tokuka A, Inomoto T, Yamaoka Y.
    Journal: Res Exp Med (Berl); 1995; 195(6):343-54. PubMed ID: 8904024.
    Abstract:
    Many of the reports implicating the contribution of oxygen radicals to preservation-reperfusion injury have been based largely on indirect experiments demonstrating the effects or the consumption of various antioxidants. Investigations based on the direct measurement of the amounts of oxygen radicals that are actually formed during reoxygenation after preservation have not given satisfactory results. In this study, we attempted direct measurement of H2O2 from hepatocellular mitochondria and superoxide (O2-) from Kupffer cells, using the HRP method and cytochrome c perfusion method, respectively, for quantitative comparison of the cold preservation-induced changes in radical generation activity between these sources. H2O2 generation in mitochondria isolated after 24 h cold preservation decreased to 8% of non-preserved liver, but in the mitochondria isolated from the livers that were reperfused for 30 min after 24 h preservation H2O2 generation recovered to 60%. The respiratory control ratio also decreased significantly after 24 h preservation, and similarly recovered after an additional 30 min reperfusion. By contrast, O2- from Kupffer cells increased in time-dependent fashion until 12 h preservation and decreased after 24 h preservation. Although 12 h preservation did not cause an increase in LDH release, the lipid peroxide in the perfusate significantly increased after 12 h preservation, which indicated the occurrence of lipid peroxidation in the sinusoidal area. These results suggested that mitochondrial H2O2 was dependent upon the activity of respiratory function and so did not cause hepatocellular injury and that O2- from Kupffer cells contributed to oxidative injury to the sinusoidal lining cells. Our data support reports demonstrating the vulnerability of nonparenchymal cells.
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