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  • Title: Binding of [125I]concanavalin A by interspecific amoeba hybrids.
    Author: Chatterjee S.
    Journal: Cell Differ; 1977 Aug; 6(2):105-10. PubMed ID: 890742.
    Abstract:
    Two types of interspecific amoeba hybrids, viz. PnIc (Amoeba proteus nucleus in A. indica cytoplasm) and InPc (A. indica nucleus in A. proteus cytoplasm), were tested for their [125I]Concanavalin A (Con A) binding activity at different periods of time. The cell surface binding of the labelled Con A was reduced to approx. 40 and 75% in the PnIc and InPc cells, respectively, 96 h after the cells were made hybrids. A significant increase in the binding of radioactive Con A was observed after the homologous nucleus was back transferred into InPc cells, but not in PnIc cells. No significant difference in the binding of the radioactive Con A was found between the progeny of the hybrid amoebae and the control amoebae. The Con A binding activity of the interspecific amoebae has been discussed in relation to the changes in the cytoplasmic membrane structures which presumably play a role in the formation of cell surface in the amoebae.
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