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  • Title: Polymerase chain reaction detection of herpes simplex virus in tear fluid from atypical herpetic epithelial keratitis after penetrating keratoplasty.
    Author: Tei M, Nishida K, Kinoshita S.
    Journal: Am J Ophthalmol; 1996 Nov; 122(5):732-5. PubMed ID: 8909218.
    Abstract:
    PURPOSE: We investigated the efficacy of polymerase chain reaction (PCR) in diagnosing atypical epithelial keratitis after a penetrating keratoplasty (PKP) for herpetic corneal scarring. METHODS: We examined three eyes of three patients with keratitis after a PKP for herpetic corneal scarring. The clinical presentation of the keratitis was atypical. DNA extracted from the tears was subjected to PCR and Southern blot hybridization. RESULTS: Herpes simplex virus DNA was identified in all cases of presumptive recurrent herpetic keratitis. CONCLUSION: Polymerase chain reaction is rapid and effective for diagnosing atypical recurrent herpetic keratitis.
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