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  • Title: Purification of a human prostate specific antigen.
    Author: Wang MC, Valenzuela LA, Murphy GP, Chu TM.
    Journal: Invest Urol; 1979 Sep; 17(2):159-63. PubMed ID: 89106.
    Abstract:
    Rabbit antiserum raised against the crude extract of normal human prostatic tissue contained antibodies to a prostatic tissue-specific antigen as shown by immunoprecipitation techniques. Using this antiserum a prostate antigen was detected in normal, benign hypertrophic, and malignant prostatic tissues, but not in other human tissues. The prostate antigen was purified to homogeneity from prostatic tissues and showed a single protein band on analytical polyacrylamide gel electrophoresis and isoelectric focusing. This report thus presents the first demonstration of the purification of a prostate-specific antigen that does not represent prostatic acid phosphatase.
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