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  • Title: Biotin-labeled antigen: a novel approach for detection of Puumala virus-specific IgM.
    Author: Ivanov A, Vapalahti O, Lankinen H, Tkachenko E, Vaheri A, Niklasson B, Lundkvist A.
    Journal: J Virol Methods; 1996 Oct; 62(1):87-92. PubMed ID: 8910652.
    Abstract:
    A novel enzyme-linked immunosorbent assay, BLA IgM-ELISA, based on baculovirus-expressed Puumala (PUU) virus nucleocapsid protein, was developed for rapid diagnosis of nephropathia epidemica. The recombinant antigen (bac-PUU-N) was purified to homogeneity by HPLC and conjugated to biotin. The biotin-streptavidin system, in combination with the mu-capture technique, rendered the BLA IgM-ELISA a sensitivity similar to or higher than that of PUU virus IgM mu-capture ELISA based on native antigen. The assay was shown to be highly specific when evaluated using a panel of 160 patient and control sera.
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