These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: Precise coexistence of regulatory peptides in the nerve fibers of the amphibian carotid labyrinth demonstrated by a combination of double immunofluorescence labelling and a multiple dye filter.
    Author: Kusakabe T, Kawakami T, Ono M, Syoui N, Kurihara K, Takenaka T, Sawada H.
    Journal: Brain Res; 1996 Oct 07; 735(2):307-10. PubMed ID: 8911669.
    Abstract:
    An application of double-immunolabelling in combination with a multiple dye filter system demonstrated new findings regarding the distribution pattern of peptidergic fibers in the carotid labyrinth to addition to our previous findings shown by the individual filter system. In high magnification images of about 10% of the yellowish fibers which represent the coexistence of two neuropeptides, there was a definite difference in localization between the fluorescence originating from rhodamine (substance P fibers) and from FITC (vasoactive intestinal polypeptide and neuropeptide Y fibers), but it was clear that they are intertwined within a single nerve bundle. This combination method was able to discriminate two different peptidergic fibers which run side by side. The coexistence suggested previously by the individual filter system may actually be due to the phenomenon described above. This means that it is necessary to apply the multiple dye filter system for reliable evidence of coexistence of different two substances in a single nerve fiber.
    [Abstract] [Full Text] [Related] [New Search]