These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: Factors affecting pentoxifylline stimulation of sperm kinematics in suspensions.
    Author: Paul M, Sumpter JP, Lindsay KS.
    Journal: Hum Reprod; 1996 Sep; 11(9):1929-35. PubMed ID: 8921066.
    Abstract:
    Sperm suspensions were prepared either by the 'swim-up' technique or by a Percoll gradient method before pentoxifylline was added to improve sperm motion characteristics. They were subsequently washed and then incubated with oocytes in in-vitro fertilization (IVF) programmes. The curvilinear velocity and lateral head displacement were approximately 20% higher in Percoll gradient-separated samples compared with samples prepared by the swim-up technique (P > 0.001), sperm motion characteristics being assessed by computer-assisted semen analysis. The dose-response study in which samples were separated by Percoll gradient/swim-up method showed that pentoxifylline gave maximum enhancement of sperm motion characteristics at a concentration of 2.8 mM/l, when the curvilinear velocity and lateral head displacement were significantly increased (P > 0.001). However, when pentoxifylline was removed by washing, the enhanced motion characteristics were reduced or lost in the process. Surprisingly, washing had a significant effect on the control samples, the motion characteristics being increased significantly (P > 0.001). This study casts doubt on the usefulness of pentoxifylline in IVF programmes.
    [Abstract] [Full Text] [Related] [New Search]