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  • Title: Comparison of immunofluorescence with enzyme immunoassay for detection of Q fever.
    Author: D'Harcourt SC, Soto AB, Burgos VC, Calero DL, Martínez-Zapico R.
    Journal: Eur J Clin Microbiol Infect Dis; 1996 Sep; 15(9):749-52. PubMed ID: 8922578.
    Abstract:
    To evaluate enzyme immunoassay (EIA) as an alternative to indirect immunofluorescence assay (IFA) to screen for Q fever in humans, 157 serum samples from patients suspected of having the disease were tested for immunoglobulin G antibodies to Coxiella burnetii. The agreement between the tests and the sensitivity of EIA were excellent (96.8% and 98.4%, respectively) when an IFA titer of > 1/160 was considered positive. All serum samples with a titer of > 1/320 in the IFA were also positive by the EIA. The EIA seems to be an acceptable alternative to IFA for screening for Q fever.
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