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  • Title: Simultaneous analysis of bases, nucleosides and nucleoside mono- and polyphosphates by high-performance liquid chromatography.
    Author: Floridi A, Palmerini CA, Fini C.
    Journal: J Chromatogr; 1977 Aug 01; 138(1):203-12. PubMed ID: 893594.
    Abstract:
    A single-column high-performance liquid chromatographic system for the simultaneous separation of bases, nucleosides and nucleoside mono- and polyphosphates has been developed, in which a strong porous anion-exchange resin (Aminex A-14) is used. The chromatographic run, carried out at 55 degrees and at alkaline pH by using a linear gradient both of ionic strength and pH, takes less than 225 min. The quantitative application of the described procedure to the analysis of cell nucleotide pools is reported.
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