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  • Title: Protein expression using ubiquitin fusion and cleavage.
    Author: Baker RT.
    Journal: Curr Opin Biotechnol; 1996 Oct; 7(5):541-6. PubMed ID: 8939629.
    Abstract:
    Expressing proteins and polypeptides as fusions to ubiquitin offers the advantage of an often dramatic increase in yield, and the ability to produce any desired amino-terminal residue upon ubiquitin cleavage. The recent availability of cloned ubiquitin-cleaving enzymes has enhanced this technique for both bacterial and eukaryotic host systems.
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