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  • Title: Facile purification of highly active recombinant Staphylococcus hyicus lipase fragment and characterization of a putative lid region.
    Author: Chang RC, Chen JC, Shaw JF.
    Journal: Biochem Biophys Res Commun; 1996 Nov 21; 228(3):774-9. PubMed ID: 8941352.
    Abstract:
    A fragment of Staphylococcus hyicus lipase gene (Ala248 to Ala640) was inserted into plasmid pET20(b+). The resulting His-tagged recombinant protein (49 kDa) was overexpressed in Escherichia coli BL21(DE3) as an highly active lipase and was purified by nickel-coupled resin. Site-directed mutagenesis showed that in comparison with wild type enzyme, the L326F and L326A enzymes showed a 3.4 and 5 fold increase in the K(m), respectively, but only a 44% and a 64% decrease in the kcat/K(m), respectively, suggesting that Leu326 of the putative lid participated in substrate-binding.
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