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  • Title: Induction of systemic murine B-cell responses by Fusobacterium nucleatum and Porphyromonas gingivalis.
    Author: Nunes IP, Jonsson R, Jensen HB, Bakken V.
    Journal: Oral Microbiol Immunol; 1996 Jun; 11(3):142-9. PubMed ID: 8941767.
    Abstract:
    The purpose of this study was to examine the antigenic abilities of Fusobacterium nucleatum strain ATCC 25586 and Porphyromonas gingivalis strain W50 black inbred BALB/cABom mice immunized subcutaneously. Furthermore, we aimed to analyze whether the outer membranes (OM) and whole cells (WC) of F. nucleatum or P. gingivalis had an effect on the levels of antibody response and whether a combination of both could either enhance or suppress the B-cell response. A single-cell assay, solid-phase enzyme-linked immunospot (ELISPOT), was used to analyze the splenic B-cell response (immunoglobulin A (IgA), IgG and IgM). Enzyme-linked immunosorbent assay (ELISA) and immunoblotting were used to verify the specific antibody response in the sera. A statistically significant lower level of spontaneous antibody production was observed in the group immunized with P. gingivalis OM compared with groups immunized with F. nucleatum and saline. The specific antibody titers measured by ELISA indicated that the bacterial preparations were able to induce IgG and IgM response. The preparations containing P. gingivalis OM induced higher humoral response than the preparations containing P. gingivalis WC, but for F. nucleatum such a difference was not observed. The prominent proteins revealed had apparent molecular masses of 40 kDa for F. nucleatum and 115, 55-56 and 43 kDa for P. gingivalis; whereas the immunoreactive proteins were 70, 65 and 40 kDa for mice immunized with F. nucleatum and 115, 55-56, 43 and 33-34 kDa for mice immunized with P. gingivalis. Quantitative analysis of B-cell response at the single cell level with ELISPOT revealed that some component(s) of P. gingivalis OM may have a suppressive ability on splenocytes incubated for a short time.
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