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  • Title: Subcellular fractionation by differential and zonal centrifugation of the trypanosomatid Crithidia fasciculata.
    Author: Edwards C, Lloyd D.
    Journal: J Gen Microbiol; 1977 Jun; 100(2):339-46. PubMed ID: 894263.
    Abstract:
    Homogenates of Crithidia fasciculata were fractionated by differential centrifugation. Mitochondria were sedimented quantitatively at 10(4) g-min and accounted for approximately 10% of the total recovered protein. Catalase was found exclusively in the supernatant fraction whilst NADH:cytochrome c oxidoreductase and p-nitrophenylphosphatase were found in all the fractions. Zonal centrifugation confirmed that catalase was non-sedimentable. Clean separation of mitochondria was obtained in both high-speed and rate zonal experiments, but no NADH:cytochrome c oxidoreductase activity could be detected in these organelles. Separation of large lysosomal vacuoles which contained p-nitrophenylphosphatase activity was obtained and these were clearly resolved from mitochondria by both high-speed and rate zonal centrifugation.
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